Publication: Histopathological changes in the small intestines of mice induced by Latana camara L. leaf extract
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Date
2005-03
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Don Mariano Marcos Memorial State University - South La Union Campus
Abstract
The study dealt with the determination of the histopathological
changes in the small intestines of the mice as induced by Lantana
camara L. leaf crude extract. This study utilized the experimental method
of research that focused on the use of standard laboratory procedure
such as extraction of plant material.
The test organisms used in the study were 54, 3- month- old, ICR
strain female mice. Six treatments were utilized namely, To - negative
control, 0.25 m1 distilled water, Tl - solvent control, 0.25 m1 PBS, T2 -
positive, control, O.20'm1 ethanol; T3 - 25 mg/kg mouse; T4 - 50 mg/kg
mouse; and Ts - 75 mg/kg mouse.
The effects of the extract observed in the small intestines of mice
both present in the duodenum and ileum were the following: blunting of
villi and/or narrowing of villi, erosion/effusion of villi, sloughing off of
villi, presence of inflammatory cells like neutrophils, macrophages,
lymphocytes and plasma cells, presence of cellular debris in the lumen,
absence of lymphatic nodules, thinning of serosa, presence of poorly
demarcated glands, necrosis of submucosa, necrotic glands and
lymphocytic infiltration around glands and krypts.
The percentage of organisms with tissue abnormalities in the
duodenum and ileum were determined by counting the number of
organisms with tissue abnormality divided by the total number of the
organisms-examined multiplied by 100. The data was subjected to the
One-Way Analysis of Variance. Results of the ANOVA showed that the
differences among the treatment means were highly significant for both
duodenum and ileum.
The Duncan's Multiple Range Test was used to test the
relationship among the treatment means. For the duodenum, results of
the DMRT showed that the treatments containing different concentration
of L. camara L. leaf crude extract, 25 mg/kg mouse, 50 mg/kg mouse
and 75 mg/kg mouse have comparable effects and were the most potent
treatments in causing. abnormalities. This was followed by the positive
control; 0.20 m1 ethanol then the negative control, 0.25 m1 distilled
water. Their effects were not comparable to each other or to the other
treatments. The solvent control, 0.25 m1 PBS had the least potent effect
in causing abnormality to the duodenum and its effect was not
comparable to the other treatments.
For the ileum, DMRT results showed that the two treatments with
the highest concentration of L. camara L. leaf crude extract, 75 mg/kg
mouse and 50 mg/kg mouse have comparable effects and were the most
potent treatments in causing abnormalities. This was followed by the
positive control, 0.20 m1 ethanol then the treatment with the lowest
concentration of the extract, 25 mgjkg lJlouse. However, their effects
were not comparable to each other or to the other treatments. The
negative and solvent controls, 0.25 m1 distilled water and 0.25 m1 PBS
have comparable effects and were the least potent in causing tissue
abnormalities-in the ileum.
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Citation
Estoesta, J. A. P. & Yaranon, K. L. (2005) Histopathological changes in the small intestines of mice induced by Latana camara L. leaf extract [Unpublished Undergraduate thesis]. Don Mariano Marcos Memorial State University - South La Union Campus, Agoo, La Union.