Publication: In vitro bulbing of garlic (Allium sativum L. var. MEXICAN) as influenced by types of culture vessels and concentrations of coconut water
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Date
2024-05
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Don Mariano Marcos Memorial State University- North La Union Campus
Abstract
The in vitro plant culture is an aseptic technique that produces original grown
garlic bulbs that are certified true-to-type and resist the disease of garlic. It was believed
that in vitro culture depends only in nutrient media therefore efforts are made to improve
the composition of culture media. The study aims to determine the effectiveness of
different types of culture vessels and coconut concentrations. The study was laid out in
Complete Randomized Design (CRD) with two factors replicated three times. It was
conducted at Plant Tissue Culture Laboratory in DMMMSU NLUC, Sapilang, Bacnotan
La Union from November 8, 2022 to April 6, 2023. Test tubes and wide mouth bottles
were used as culture vessels, with coconut water concentrations of 0%, 10%, and 20%.
The results showed that the different types of culture vessel significantly affected
the number of days to bulbing, maturity, and contamination. Test tubes proved to be more
efficient, taking a shorter time to bulb and mature than the wide-mouthed bottles, thereby
reducing the risk of contamination. Additionally, the addition of coconut water at 20%
concentration resulted in the highest number of garlic roots and heavy bulblets.
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Citation
Macadenden, C.J.B. (2024). In vitro bulbing of garlic (Allium sativum L. var. MEXICAN) as influenced by types of culture vessels and concentrations of coconut water. [Unpublished Undergraduate Thesis, Don Mariano Marcos Memorial State University- North La Union Campus, Bacnotan, La Union].