Publication: The potential of sweet potato (Ipomea batatas) as laboratory culture media for fungi
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2008-03
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Don Mariano Marcos Memorial State University - North La Union Campus
Abstract
This study was conducted to: a) determine the efficacy of sweet potato utilized as culture media for fungi; b) determine the most effective concentration of sweet potato utilized as culture media for fungi; c) determine the variety of sweet potato that best supports the growth of fungi; and d) determine the economic feasibility of using sweet potato agar media in the cultivation of fungi.
The Complete Randomized Design (CRD) considering three factors was used in the study. The factors were as follows: Factor A (Variety of sweet potato) UPL Sp 2 (V1), V Sp 2 (V2) and UPL Sp 5 (V3); Factor B (Concentration of sweet potato), 75% (C1), 85% (C2) and 95% (C3); and Factor C (Species of fungi), Candida albicans (F1), Aspergillus niger (F2) and Microsporum canis (F3).
Sweet potato tubers were washed, peeled, ground, extracted, oven-dried, pulverized and combined with dextrose, agar powder and water to form a media. Each prepared culture plate was inoculated with the selected fungi and incubated at their required temperatures and periods. Colony size was measured after their incubation period. The data gathered were analyzed using Analysis of Variance (ANOVA).
Results showed that sweet potato agar media has a high potential for the laboratory cultivation of Candida albicans, Aspergillus niger and Microsporum canis. Concentrations of 75, 85 and 95 percent of sweet potato dextrose agar are effective on the culture of Candida albicans and Microsporum canis. For Aspergillus niger the
concentrations of 85 and 95% are more effective than 75% concentration. The UPL Sp 2 (V1), V Sp 2 (V2) and UPL Sp 5 (V3) varieties of sweet potato can be prepared as a culture media for the cultivation of Candida albicans, Aspergillus niger and Microsporum canis. Producing sweet potato agar media at 75%, 85%, and 95%
concentration was economical.
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Citation
Garcia, J. M. (2008). The potential of sweet potato (Ipomea batatas) as laboratory culture media for fungi. [Unpublished Undergraduate Thesis]. Don Mariano Marcos Memorial State University - North La Union Campus, Sapilang, Bacnotan, La Union. Lakasa ti Sirib, DMMMSU Institutional Repository.