Publication: Comparison of the sperm viability, morphology, and morphometry of fresh and frozen-thawed semen of water buffaloes
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Date
2020-05
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Don Mariano Marcos Memorial State University - North La Union Campus
Abstract
Semen with high number of live and normal spermatozoa is a good indicator of fertility. This study was conducted on fresh and frozen-thawed semen of water buffalo bulls used in AI to: (a) determine and compare their sperm viability; (b) determine and compare their sperm morphology; (c) determine and compare their sperm morphometry and; (d) determine the effects of cryopreservation on these sperm parameters.
Five fresh and seven frozen-thawed semen samples were used in the study. A microscope with Nikon imaging software and NIS elements application installed were used to evaluate the sperm parameters.
All the data gathered were reported as mean ± s.e.m and significant differences were statistically analyzed by T-test at P<0.05.
Fresh semen samples have a significantly higher (p<0.05) number in live and normal spermatozoa. Sperm head on the frozen-thawed semen samples were larger. In these given results, cryopreservation greatly affects the sperm parameters in the study.
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Gacayan, M. E. (2020). Comparison of the sperm viability, morphology, and morphometry of fresh and frozen-thawed semen of water buffaloes. [Unpublished Undergraduate Thesis]. Don Mariano Marcos Memorial State University - North La Union Campus, Sapilang, Bacnotan, La Union. Lakasa ti Sirib, DMMMSU Institutional Repository.