Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

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creativework.keywordsAgriculture, Genetics, Microbiology
dc.contributor.authorMason, Maria Luisa T.
dc.contributor.authorTabing, Baby Lyn C.
dc.contributor.authorYamamoto, Akihiro
dc.contributor.authorSaeki, Yuichi
dc.date.accessioned2026-08-20T02:19:29Z
dc.date.available2026-08-20T02:19:29Z
dc.date.issued2018
dc.descriptionFull text.
dc.description.abstractOne of the strategies that is commonly used in the Philippines to improve the production of soybean is by inoculation. However, this technique often fails mainly due to the lack of information about the indigenous soybean rhizobia in the Philippines soil. In this study, the diversity of indigenous bradyrhizobia collected from the non-flooded and flooded soil conditions at 11 locations in the country was investigated using a local soybean cultivar as the host plant. The genetic variation among the 424 isolates was detected through Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) treatment and sequence analysis for 16S rRNA gene, 16S-23S rRNA internal transcribed spacer (ITS) region and rpoB housekeeping gene. All the isolates were classified under the Bradyrhizobium species namely B. elkanii, B. diazoefficiens, B. japonicum, B. yuanmingense and a considerable proportion of the isolates were clustered under Bradyrhizobium sp. The isolates which were classified under Bradyrhizobium sp. were thought to be endemic to Philippines soil as evidenced by their nucleotide divergence against the known rhizobia and the historical absence of rhizobia inoculation in the collection sites. The major influence on the distribution and diversity of soybean bradyrhizobia is attributed to the difference in the flooding period, followed by soil properties such as pH, soil type, and nutrient content. As determined, it is proposed that the major micro-symbiont of soybean in the Philippines are B. elkanii for non-flooded soils, then B. diazoefficiens and B. japonicum for flooded soils. © 2018
dc.identifier.citationMason, M. L. T., Tabing, B. L. C., Yamamoto, A., & Saeki, Y. (2018). Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines. Heliyon, 4(11). https://doi.org/10.1016/j.heliyon.2018.e00921
dc.identifier.issn2405-8440
dc.identifier.urihttps://lakasa.dmmmsu.edu.ph/handle/123456789/2402
dc.language.isoen
dc.publisherElsevier Ltd
dc.sdgSDG 2
dc.subjectSoil microbiology
dc.subjectBradyrhizobium
dc.subjectSoybean
dc.subjectGenetic diversity
dc.subjectPhilippines
dc.subject.ddc631.4
dc.subject.lcshSoil microbiology
dc.subject.lcshBradyrhizobium--Genetics
dc.subject.lcshSoybean--Nitrogen fixation
dc.titleInfluence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines
dc.typeArticle
oaire.citation.issue11
oaire.citation.volume4
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