In vitro antitumor properties of an isolate from leaves of Cassia alata L

Simple item page
creativework.keywordsCassia alata L, in vitro antitumor property - MTT formazan assay - TUNEL - caspase assay
dc.contributor.authorOlarte, Elizabeth Iglesias
dc.contributor.authorHerrera, Annabelle Aliga
dc.contributor.authorVillaseñor, Irene Manese
dc.contributor.authorJacinto, Sonia Donaldo
dc.date.accessioned2026-08-19T16:41:18Z
dc.date.available2026-08-19T16:41:18Z
dc.date.issued2013
dc.descriptionFull text
dc.description.abstractLeaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a nonsmall cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with IC50 values of 16, 17, and 17 µg/ml, respectively. Isolate f6l, however, showed no cytotoxicity towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical investigation using DAPI staining and biochemical investigation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method used to detect DNA fragmentation-together with caspase assay, demonstrated apoptotic cell death. Spectral characterization of isolate f6l revealed that it contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile and its mode of action, f6l might represent a new promising compound with potential for development as an anticancer drug with low or no toxicity to non-cancer cells used in this study.
dc.identifier.citationOlarte, E. I., Herrera, A. A., Villaseñor, I. M., & Jacinto, S. D. (2013). In vitro antitumor properties of an isolate from leaves of Cassia alata L. Asian Pacific Journal of Cancer Prevention, 14(5), 3191–3196. https://doi.org/10.7314/APJCP.2013.14.5.3191
dc.identifier.doihttps://doi.org/10.7314/APJCP.2013.14.5.3191
dc.identifier.issn15137368
dc.identifier.urihttps://lakasa.dmmmsu.edu.ph/handle/123456789/2393
dc.language.isoen
dc.publisherAsian Pacific Journal of Cancer Prevention
dc.sdgSDG 3
dc.sdgSDG 15
dc.subjectCassia alata
dc.subjectIn vitro cytotoxicity
dc.subjectAntitumor properties
dc.subjectApoptosis
dc.subjectMTT assay
dc.subjectPolyunsaturated fatty acid esters
dc.subjectCancer cell lines (MCF-7, T24, Col 2)
dc.subject.ddcPharmacognosy
dc.subject.ddcAntineoplastic drug therapy
dc.subject.ddcPathology
dc.subject.lcshSenna alata -- Therapeutic use
dc.subject.lcshMateria medica, Vegetable -- Philippines
dc.subject.lcshAntineoplastic agents -- In vitro testing
dc.subject.lcshCancer -- Alternative treatment
dc.subject.lcshApoptosis -- Physiological aspects
dc.subject.lcshFatty acids, Unsaturated -- Therapeutic use
dc.titleIn vitro antitumor properties of an isolate from leaves of Cassia alata L
dc.typeArticle
oaire.citation.endPage3196
oaire.citation.issue5
oaire.citation.startPage3191
oaire.citation.volume14
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PUB-JAPE-SLUC-2013-OlatreEI-FT.pdf
Size:
1.38 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Journal articles published externally