Publication: Characterization and in-vitro Biomedical application: Carbon nanodots synthesized from Brassica oleracea (L.) Leaf wastes against mutltidrug resistant Esβl producing Escherichia coli
| dc.contributor.author | Tamao, Jay Ar A. | |
| dc.contributor.author | Bambao, Alyson Andrey C. | |
| dc.contributor.author | Aromin, Charlene Venice G. | |
| dc.date.accessioned | 2025-06-25T08:23:24Z | |
| dc.date.available | 2025-06-25T08:23:24Z | |
| dc.date.issued | 2024-05 | |
| dc.description.abstract | Carbon dots are a new type of carbon nanomaterial that has a size <100nm. The CD has an interesting application in the biomedical field, but the lack of an approach hinders its application in the field. Moreover, bacterial infections are attracting significant attention for the research and development of new antimicrobial agents. As one of the quinolones, CPX has a broad spectrum and strong antibacterial effect. This study used Brassica oleracea (L.) waste leaf to synthesize CDs via green synthesis and a hydrothermal approach. Surface passivation of CPX was done to improve its antibacterial performance and asses the Brassica-CDs biocompatibility. The particles were characterized using UV Vis spectrophotometry, FTIR, DLS, and SEM to elucidate their in-vitro application against MDR ESBL E. coli. Results yielded a green emitting color under 395nm; optical properties showed π-π* transition of Brassica-CD and 351nm n-π* transition with a tail extending for B-CDCPX. ζ potential and particle size showed 8.65mV, and an increase of 9.68mV indicates a slightly positive charge, which correlates to the size increase of particles from 93.05 to 153nm having an amorphous size distribution of Brassica-CD. Meanwhile, functional groups showed active groups O–H, C=C, C≡C, C=O with attachment of C–N, C–F, and C–H for conjugated Brassica-CD. Interestingly, B-CDCPX has a better antibacterial activity than Brassica-CD, CPX, and AMP due to the double synergistic effect, which contributed to its excellent antibacterial activity against MDR ESBL E. coli. Therefore, novel Brassica-CD and modified B-CDCPX with a broad-spectrum antibacterial activity will provide a platform for antibacterial nanomaterial for potential application in the future of biomedical, pharmaceutical, and medications. | |
| dc.identifier.citation | Tamayo, J.A., Bambao, A.C., Aromin, C.G. (2024). Characterization and in-vitro Biomedical application: Carbon nanodots synthesized from Brassica oleracea (L.) Leaf wastes against mutltidrug resistant Esβl producing Escherichia coli [Unpublished Undergraduate Thesis] Don Mariano Marcos Memorial State University - North La Union Campus, Sapilang, Bacnotan, La Union. DMMMSU Institutional Repository. | |
| dc.identifier.uri | https://lakasa.dmmmsu.edu.ph/handle/123456789/165 | |
| dc.publisher | Don Mariano Marcos Memorial State University - North La Union Campus | |
| dc.title | Characterization and in-vitro Biomedical application: Carbon nanodots synthesized from Brassica oleracea (L.) Leaf wastes against mutltidrug resistant Esβl producing Escherichia coli | |
| dc.type | Thesis | |
| dspace.entity.type | Publication |