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Molecular detection of african swine fever in oronasal samples from slaughterhouses in the first district of La Union

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dc.contributor.authorCañero, Etheline P.
dc.date.accessioned2026-02-11T01:58:00Z
dc.date.available2026-02-11T01:58:00Z
dc.date.issued2025-05
dc.description.abstractDue to the significant impact of African Swine Fever, particularly on food security, pork production, and the economy, this study aimed to assess the prevalence of African Swine Fever (ASF) in the First District of La Union using qRT-PCR. Data on the total pig population slaughtered was gathered from the Department of Agriculture in each municipality of the district. The sample size was determined using the Lynch Formula, and a total of 62 samples were collected for analysis. DNA was extracted using the Wizard® Genomic DNA Purification Kit. The DNA concentration was measured using a NanoDrop UV-Vis Spectrophotometer. The DNA quality was assessed through a gel documentation system, and the oronasal samples were tested for ASF using the DLAB Accurate 96 system. The results showed no presence of ASF detected in the oronasal samples from the First District of La Union. Thus, indicating a 0% prevalence rate of the disease in the area.
dc.identifier.citationCañero, E. P. (2025). Pain management is a critical aspect of veterinary care for companion animals. This study aimed to assess the knowledge, attitudes, and practices (KAP) of companion animal veterinarians in La Union regarding pain management. Using a descriptive cross-sectional design, data were gathered from 30 licensed veterinarians using validated, semi-structured questionnaire. The study examined respondents' demographic profiles, familiarity with pain assessment tools, perceived barriers, and resource needs. Results revealed that while most veterinarians recognized the importance of pain management, only 10% were highly familiar with formal pain assessment tools. Although 96.7% acknowledged its significance, challenges such as treatment costs (86.7%), client compliance (76.7%), and pain recognition (70%,), hindered implementation. Moreover, 30% had no formal training in pain management, and 56.7% lacked access to updated guidelines. These findings underscore the need for improved education, resources, and support to enhance pain management practices among veterinarians in the region. [Unpublished Undergraduate Thesis]. Don Mariano Marcos Memorial State University - North La Union Campus, Sapilang, Bacnotan, La Union. Lakasa ti Sirib, DMMMSU Institutional Repository.
dc.identifier.urihttps://lakasa.dmmmsu.edu.ph/handle/123456789/1010
dc.language.isoen_US
dc.publisherDon Mariano Marcos Memorial State University - North La Union Campus
dc.sdgSDG 6
dc.titleMolecular detection of african swine fever in oronasal samples from slaughterhouses in the first district of La Union
dc.typeThesis
dspace.entity.typePublication
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