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Molecular detection of african swine fever in oronasal samples from slaughterhouses in the first district of La Union

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dc.contributor.authorCañero, Etheline P.
dc.date.accessioned2026-06-10T01:03:28Z
dc.date.available2026-06-10T01:03:28Z
dc.date.issued2025-05
dc.description.abstractDue to the significant impact of African Swine Fever, particularly on food security, pork production, and the economy, this study aimed to assess the prevalence of African Swine Fever (ASF) in the First District of La Union using qRT-PCR. Data on the total pig population slaughtered was gathered from the Department of Agriculture in each municipality of the district. The sample size was determined using the Lynch Formula, and a total of 62 samples were collected for analysis. DNA was extracted using the Wizard® Genomic DNA Purification Kit. The DNA concentration was measured using a NanoDrop UV-Vis Spectrophotometer. The DNA quality was assessed through a gel documentation system, and the oronasal samples were tested for ASF using the DLAB Accurate 96 system. The results showed no presence of ASF detected in the oronasal samples from the First District of La Union. Thus, indicating a 0% prevalence rate of the disease in the area.
dc.identifier.citationCañero, E. P. (2025). Molecular detection of african swine fever in oronasal samples from slaughterhouses in the first district of La Union. [Unpublished Undergraduate Thesis]. Don Mariano Marcos Memorial State University - North La Union Campus, Sapilang, Bacnotan, La Union. Lakasa ti Sirib, DMMMSU Institutional Repository.
dc.identifier.urihttps://lakasa.dmmmsu.edu.ph/handle/123456789/1805
dc.language.isoen_US
dc.publisherDon Mariano Marcos Memorial State University - North La Union Campus
dc.sdgSDG 2
dc.titleMolecular detection of african swine fever in oronasal samples from slaughterhouses in the first district of La Union
dc.typeThesis
dspace.entity.typePublication
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