LAKASABrowsing by Author "Orine, Kimberly G."

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Molecular detection of African swine fever in blood samples from slaughterhouses in District 1, La Union
(Don Mariano Marcos Memorial State University North La Union Campus, 2025-04) Orine, Kimberly G.
African Swine Fever (ASF) poses severe economic and agricultural challenges due to its high mortality rate and the absence of an effective vaccine. This study detects the prevalence of ASF in District 1 of La Union using quantitative real-time PCR (qRT-PCR) as the diagnostic tool. Data on the total slaughtered pigs from the municipalities of District 1, La Union were obtained from the Department of Agriculture. Sixty-two (62) pig blood samples were collected. Extraction of DNA were performed using the Wizard® Genomic DNA Purification Kit and TaqMan qPCR assay. Prevalence rate was calculated by dividing the ASF positive samples by the number of samples, then multiplying the result by one hundred (100). The findings revealed the number of daily slaughtered pigs after the emergence of ASF. There was zero percent (0%) ASF prevalence rate among the pigs. Consequently, an epidemiological map could not be developed.