LAKASABrowsing by Author "Bustillos, Danelle A."

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Detecting african swine fever using real-time polymerase chain reaction in selected meatstalls in the first district of La Union
(Don Mariano Marcos Memorial State University - North La Union Campus, 2025-11) Bustillos, Danelle A.
This study aimed to detect the presence of African Swine Fever Virus (ASFV) in environmental swab samples collected from meat stalls in the First District of La Union, recognizing the critical threat ASF poses to swine production, food security, and local economies. A cross-sectional study design was employed, and a total of 79 environmental samples were collected from meat surfaces, knives, and weighing scales across selected municipalities. The sample size was determined using the Lynch Formula at a 95% confidence level, and sample allocation was based on the number of registered meat stalls per municipality. DNA was extracted using the Wizard® Genomic DNA Purification Kit, while DNA concentration and purity were measured through a NanoDrop UV-Vis Spectrophotometer. The quality of the extracted DNA was assessed via gel electrophoresis. ASFV detection was carried out using quantitative real-time PCR (qRT-PCR) with the DLAB Accurate 96 system. Results showed that all environmental samples tested negative for ASFV, indicating a 0% prevalence rate within the study area. These findings suggest an absence of ASFV contamination in the sampled meat stalls, reflecting the possible effectiveness of current sanitation and biosecurity measures in place.